Dna 분리 방법

A fast, integrated workflow for a wide range of applications, from human whole-genome sequencing to amplicons, plasmids, and microbial species. Read More...

Library Prep

Illumina® DNA Prep, (M) Tagmentation (24 Samples, IPB)

20060060

Illumina® DNA Prep, (M) Tagmentation (96 Samples, IPB)

20060059

Illumina® DNA Prep, (M) Tagmentation (24 Samples)

20018704

Illumina® DNA Prep, (M) Tagmentation (96 Samples)

20018705

Index Adapters

IDT® for Illumina® DNA/RNA UD Indexes Set A, Tagmentation (96 Indexes, 96 Samples)

20027213

IDT® for Illumina® DNA/RNA UD Indexes Set B, Tagmentation (96 Indexes, 96 Samples)

20027214

IDT® for Illumina® DNA/RNA UD Indexes Set C, Tagmentation (96 Indexes, 96 Samples)

20042666

IDT® for Illumina® DNA/RNA UD Indexes Set D, Tagmentation (96 Indexes, 96 Samples)

20042667

IDT® for Illumina Nextera DNA Unique Dual Indexes Set C (96 Indexes, 96 Samples)

20027215

IDT® for Illumina Nextera DNA Unique Dual Indexes Set D (96 Indexes, 96 Samples)

20027216

Nextera™ DNA CD Indexes (24 Indexes, 24 Samples)

20018707

Nextera™ DNA CD Indexes (96 Indexes, 96 Samples)

20018708

Services

Illumina DNA Prep Training

20022900

Illumina Purification Bead, 100mL

20060057

Illumina Purification Bead, 400mL

20060058

Flex Lysis Reagent Kit (96 reactions)

20018706

Illumina® Free Adapter Blocking Reagent (12 Reactions)

20024144

Illumina® Free Adapter Blocking Reagent (48 Reactions)

20024145

NextSeq PhiX Control Kit

FC-110-3002

Product Highlights

Illumina DNA Prep offers flexibility for many whole-genome sequencing applications.

Fastest Illumina library prep workflow, with ~3.5 hours total time
Flexibility to accommodate variations in sample type, DNA input amount, and application
Optimized library prep performance, generating reliable results

Learn more about the technology

Save Time and Resources

Illumina DNA Prep uses a fast, user-friendly workflow. On-bead tagmentation chemistry reduces total library prep time to ~3.5 hours, from DNA extraction to library normalization.

Simplify Lab Operations

The Illumina DNA Prep workflow supports a broad DNA input range (1–500 ng), multiple sample types, and both small and large genomes. The workflow includes DNA extraction from blood, saliva, or dried blood spots*or bacterial colonies*.

Gain the flexibility to sequence human or other large, complex genomes as well as amplicons or microbial species, all with a single kit.

Obtain Reliable Results

While accommodating various study requirements, the Illumina DNA Prep workflow delivers consistent insert sizes, uniform coverage, and optimized performance, regardless of DNA input amount or genome size. The bead-based technology minimizes bias and opportunities for error, resulting in highly reproducible sequencing data.

Access Flexible Throughput Options

The IDT for Illumina - DNA/RNA UD Indexes Sets A, B, C, and D offer up to 384 unique dual indexes, enabling accurate assignment of reads and efficient use of the flow cell. (Sets A and B are available now. Sets C and D are coming soon.) These unique dual index codes use 10 base pair codes. This change in base pair index codes require adjustments to the sequencing run setup.

Nextera DNA CD Indexes support up to 96-sample combinatorial dual indexing. The 24 CD Indexes are supplied in a tube format, and 96 in a plate format.

Related Notification

For whole-genome sequencing, Illumina DNA Prep is the recommended replacement for the Nextera DNA Library Prep Kit, which has been discontinued. If you were using Nextera DNA (Cat. No. FC-121-1030) or stand-alone components (Cat. Nos. 15027865 and 15027866) for ATAC-Seq** or other custom applications, the Illumina Tagment DNA TDE1 Enzyme and Buffer Kit is our suggested alternative.

*Demonstrated protocols available.

**Customer-reported application

Explore Illumina Library Prep

Discover the power that Illumina library prep tagmentation technology can bring to your lab through this interactive experience. Our library prep solutions offer whole-genome sequencing across various applications, flexibility across Illumina sequencers, and integrated sample input for blood and saliva without quantification.

View Infographic

Specifications

Project Recommendations

Instrument	Recommended Number of Samples	Read Length
NextSeq 550 System	1 sample per run (high output; based on 30× coverage of a human genome)	Up to 2 × 150 bp
NovaSeq 6000 System	2–10 samples per run (dual flow cell; based on 30× coverage of a human genome)	Up to 2 × 125 bp (rapid run) Up to 2 × 150 bp (high output)

Product Comparison

	Illumina DNA Prep	TruSeq DNA PCR-Free	TruSeq DNA Nano
Assay Time	~3-4 hours (from DNA extraction to normalized library)	5 hours total assay time	~6 hours total assay time
Automation Capability	Liquid Handling Robots	Liquid Handling Robots	Liquid Handling Robots
Description	A fast, flexible workflow for a wide range of research applications and sample types, from human to microbial whole-genome sequencing and more.	A simple, all-inclusive PCR-free prep for whole-genome sequencing studies with the ability to sequence through challenging regions of the genome.	A low-input research method that delivers high genome coverage quality and reduced bias.
Hands-On Time	1-1.5 hours	4 hours	~4 hours
Input Quantity	Small genomes (e.g. microbial): 1-500 ng DNA. Large genomes (e.g. human): 100-500 ng DNA. (For blood and saliva, see the reference guide).	1 ug DNA	100 ng genomic DNA
Mechanism of Action	Bead-linked transposome	Mechanical DNA fragmentation and adapter ligation. Workflow is PCR-free and gel-free.	Mechanical DNA fragmentation and adapter ligation. Workflow uses reduced-bias PCR and is gel-free.
Method	Amplicon Sequencing, De Novo Sequencing, Shotgun Sequencing, Whole-Genome Sequencing	Genotyping by Sequencing, Shotgun Sequencing, Whole-Genome Sequencing	Genotyping by Sequencing, Shotgun Sequencing, Whole-Genome Sequencing
Multiplexing	Up to 384 unique dual (UD) combinations and 96 combinatorial dual (CD) combinations	Up to 24 single, 96 combinatorial (CD) dual, 24 unique dual and 96 unique dual (UD) combinations (available soon)	Up to 24 single, 96 combinatorial (CD) dual, 24 unique dual and 96 unique dual (UD) combinations (available soon)
Specialized Sample Types	Blood, Not FFPE-Validated, Saliva	Not FFPE-Compatible	Low-Input Samples, Not FFPE-Compatible
Species Category	Any Species, Bacteria, Drosophila, Human, Mammalian, Mouse, Nematode, Plant, Rat, Virus, Yeast, Zebrafish	Human, Mammalian, Mouse, Other, Plant, Rat	Human, Mammalian, Mouse, Other, Plant, Rat
Species Details	Compatible with any species	Compatible with most large DNA genomes.	Compatible with most large DNA genomes.
Target Insert Size	~350bp	350 bp or 550 bp	350 bp or 550 bp

Illumina Bead-Linked Transposome Chemistry

(A) Bead-linked transposomes mediate the simultaneous fragmentation of gDNA and the addition of Illumina sequencing primers. (B) Reduced-cycle PCR amplification amplifies sequencing ready DNA fragments and adds indexes and adapters. (C) Sequencing-ready fragments are washed and pooled.